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顶部生物活性储存和溶解信息计算器

Anti-p150 Antibody (3W818)

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Anti-p150 Antibody (3W818)
产品编号 TMAC-02934
别名 P 150, MGC71229, hp150, DCAF1, Chromatin assembly factor I p150 subunit, Chromatin assembly factor I 150 kDa, Chromatin assembly factor I (150 kDa), Chromatin Assembly Factor 1 Subunit A p150, Chromatin assembly factor 1 subunit A, CHAF1A, CAF-I p150, CAF-I 150 kDa subunit, CAF1P150, CAF1B, CAF1A_HUMAN, CAF-1 subunit A, CAF1, CAF I p150, CAF I 150 kDa subunit, CAF 1 subunit A, CAF 1, CAF

Anti-p150 Antibody (3W818) 是一种 Rabbit 抗体，靶向 p150。Anti-p150 Antibody (3W818) 可用于 WB,ICC,IF,IP,IHC,FCM。

Anti-p150 Antibody (3W818)

Anti-p150 Antibody (3W818)

Rating icon 还可以
Anti-p150 Antibody (3W818)
产品编号 TMAC-02934 别名 P 150, MGC71229, hp150, DCAF1, Chromatin assembly factor I p150 subunit, Chromatin assembly factor I 150 kDa, Chromatin assembly factor I (150 kDa), Chromatin Assembly Factor 1 Subunit A p150, Chromatin assembly factor 1 subunit A, CHAF1A, CAF-I p150, CAF-I 150 kDa subunit, CAF1P150, CAF1B, CAF1A_HUMAN, CAF-1 subunit A, CAF1, CAF I p150, CAF I 150 kDa subunit, CAF 1 subunit A, CAF 1, CAF

Anti-p150 Antibody (3W818) 是一种 Rabbit 抗体，靶向 p150。Anti-p150 Antibody (3W818) 可用于 WB,ICC,IF,IP,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,485
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-p150 Antibody (3W818) is a Rabbit antibody targeting p150. Anti-p150 Antibody (3W818) can be used in WB,ICC,IF,IP,IHC,FCM.
别名P 150, MGC71229, hp150, DCAF1, Chromatin assembly factor I p150 subunit, Chromatin assembly factor I 150 kDa, Chromatin assembly factor I (150 kDa), Chromatin Assembly Factor 1 Subunit A p150, Chromatin assembly factor 1 subunit A, CHAF1A, CAF-I p150, CAF-I 150 kDa subunit, CAF1P150, CAF1B, CAF1A_HUMAN, CAF-1 subunit A, CAF1, CAF I p150, CAF I 150 kDa subunit, CAF 1 subunit A, CAF 1, CAF
Ig Type
IgG
交叉反应
Human
验证活性
1. Western blot analysis of p150 CAF1 on K562 (1) and A431 (2) cell lysate using anti-p150 CAF1 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p150 CAF1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p150 CAF1 antibody. Counter stained with hematoxylin.
4. ICC staining p150 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining p150 CAF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining p150 CAF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of Daudi cells with p150 CAF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
应用
WB
ICC
IF
IP
IHC
FCM
推荐剂量
WB: 1:500-2000; IHC: 1:50-200; ICC:IF: 1:50-200; IP: 1:10-50; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated Histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: human p150 CAF1 aa 100-300
抗原种属
human
Uniprot ID
Q13111
化学信息
分子量Theoretical: 107 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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