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Anti-ox-LDL Polyclonal Antibody

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Anti-ox-LDL Polyclonal Antibody
产品编号 TMAB-01308

Anti-ox-LDL Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ox-LDL。Anti-ox-LDL Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF,FCM。

Anti-ox-LDL Polyclonal Antibody

Anti-ox-LDL Polyclonal Antibody

Rating icon 还可以
Anti-ox-LDL Polyclonal Antibody
产品编号 TMAB-01308

Anti-ox-LDL Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ox-LDL。Anti-ox-LDL Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF,FCM。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,960
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-ox-LDL Polyclonal Antibody is a Rabbit antibody targeting ox-LDL. Anti-ox-LDL Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
交叉反应
Human
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ox-LDL) Polyclonal Antibody, Unconjugated (TMAB-01308) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Blank control: A431. Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (TMAB-01308) Dilution: 3 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3 μg/test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (TMAB-01308)
Dilution: 2 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
应用
推荐剂量
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Low-density lipoprotein oxidised with Cu2SO4.
抗原信息
免疫原
Full length protein from human plasma
抗原种属
Human
基因ID
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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