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Anti-ORP150 Antibody (6Q346)
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产品编号 TMAC-02925
别名 ORP-150, ORP150, hypoxia up-regulated 1, HSP12A, GRP-170, Grp170
Anti-ORP150 Antibody (6Q346) 是一种 Rabbit 抗体，靶向 ORP150。Anti-ORP150 Antibody (6Q346) 可用于 WB,IHC,FCM。
Anti-ORP150 Antibody (6Q346)
Anti-ORP150 Antibody (6Q346)
还可以产品编号 TMAC-02925 别名 ORP-150, ORP150, hypoxia up-regulated 1, HSP12A, GRP-170, Grp170
Anti-ORP150 Antibody (6Q346) 是一种 Rabbit 抗体，靶向 ORP150。Anti-ORP150 Antibody (6Q346) 可用于 WB,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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化学信息
| 产品描述 | Anti-ORP150 Antibody (6Q346) is a Rabbit antibody targeting ORP150. Anti-ORP150 Antibody (6Q346) can be used in WB,IHC,FCM. |
| 别名 | ORP-150, ORP150, hypoxia up-regulated 1, HSP12A, GRP-170, Grp170 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of ORP150 on mouse prostate tissue lysate using anti-ORP150 antibody at 1/5,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ORP150 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-ORP150 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin. 6. Flow cytometric analysis of HepG2 cells with ORP150 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:5000-10000; IHC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | ORP150, also known as HYOU1 (hypoxia upregulated 1), is a 999 amino acid protein that localizes to the lumen of the endoplasmic reticulum (ER) and is a member of the heat shock protein 70 (HSP 70) family. ORP150 is highly expressed in tissues that have well-developed ERs and a large number of secretory proteins (such as liver and pancreas). It is expressed at lower levels in kidney and brain, and plays an essential role in cytoprotective cellular responses to hypoxia (oxygen deprivation). Specifically, ORP150 participates in protein folding and secretion in the ER and functions to protect cells from hypoxia-induced apoptosis, thereby playing a crucial role in cell survival. ORP150 expression is upregulated in a variety of tumors, such as breast cancer, suggesting an important role in tumorigenesis. The gene encoding ORP150 has two translation initiation sites, resulting in a truncated transcript that lacks an ER signal peptide, but is thought to function as a housekeeping protein in the cytoplasm. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: human ORP150 aa 800-950 |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 111 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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