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顶部生物活性储存和溶解信息计算器

Anti-OCT4 Antibody (4Q101)

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Anti-OCT4 Antibody (4Q101)
产品编号 TMAC-02915

Anti-OCT4 Antibody (4Q101) 是一种 Rabbit 抗体，靶向 45934。Anti-OCT4 Antibody (4Q101) 可用于 WB,ICC/IF,IHC,IP,ChIP。

Anti-OCT4 Antibody (4Q101)

Anti-OCT4 Antibody (4Q101)

Rating icon 还可以
Anti-OCT4 Antibody (4Q101)
产品编号 TMAC-02915

Anti-OCT4 Antibody (4Q101) 是一种 Rabbit 抗体，靶向 45934。Anti-OCT4 Antibody (4Q101) 可用于 WB,ICC/IF,IHC,IP,ChIP。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-OCT4 Antibody (4Q101) is a Rabbit antibody targeting 45934. Anti-OCT4 Antibody (4Q101) can be used in WB,ICC/IF,IHC,IP,ChIP.
Ig Type
IgG
交叉反应
Human,Mouse
验证活性
1. Western blot analysis of Oct4 on different lysates using anti-Oct4 antibody at 1/1,000 dilution. Positive control: Lane 1: F9, Lane 2: NCCIT, Lane 3: hES.
2. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Oct4 antibody. Counter stained with hematoxylin.
3. ICC staining Oct4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Oct4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
WB
ICC/IF
IHC
IP
ChIP
推荐剂量
WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景POU5F1 (POU domain, class 5, transcription factor 1), also known as octamer-binding transcription factor-3 (Oct-3, OTF3), octamer-binding transcription factor-4 (Oct-4, Otf-4) and Oct-3/4, modulates embryonic stem (ES) cell populations by influencing lineage commitment. Oct-3/4 sustains stem-cell self-renewal and differentiation pathways. Transcription factors containing the POU homeodomain regulate tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Oct-3/4 is capable of inducing rapid proliferation and tumorigenic properties of ES cells through activation of the UTF1 gene. In humans, two Oct-3/4 isoforms contribute to influencing the undifferentiated phenotype of ES cells. Oct-3/4 pseudogenes localizing to human chromosomes 10 and 8 are reported to be transcribed in certain cancer cell lines and tissues.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
Q01860
化学信息
分子量Theoretical: 39 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

transcription factor 1POU5F1POU5F 1POU domain, class 5, transcription factor 1OTF-3OTF3OTF 3Octamer-binding transcription factor 3 (OTF-3)Octamer-binding protein 4 (Oct-4)Octamer-binding protein 3 (Oct-3)class5class 54593445933

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