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Anti-Oct4 Antibody (4H630)
还可以产品编号 TMAY-00055
Anti-Oct4 Antibody (4H630) 是一种 Mouse 抗体，靶向 Oct4。Anti-Oct4 Antibody (4H630) 可用于 FCM。
Anti-Oct4 Antibody (4H630)
Anti-Oct4 Antibody (4H630)
还可以产品编号 TMAY-00055
Anti-Oct4 Antibody (4H630) 是一种 Mouse 抗体，靶向 Oct4。Anti-Oct4 Antibody (4H630) 可用于 FCM。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Oct4 Antibody (4H630) is a Mouse antibody targeting Oct4. Anti-Oct4 Antibody (4H630) can be used in FCM. |
| Ig Type | Monoclonal Mouse IgG2a |
| 克隆号 | 4H630 |
| 交叉反应 | Human |
| 验证活性 | Flow cytometric analysis of Oct4 in human embryonic stem (ES) cells. Human embryonic stem (ES) cells were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization buffer (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-Oct4 monoclonal antibody, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | FCM |
| 推荐剂量 | FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The POU5F1 gene, which encodes the POU domain, class 5, transcription factor 1 (also known as Oct-4), is expressed in embryonic stem cells where it regulates pluripotency and proliferation.The POU5F1 gene encodes one of the "core" transcription factors necessary to establish and maintain pluripotency in mammals. Its function depends on its precise level of expression, so its transcription has to be tightly regulated. POU5F1 is a key regulator of self-renewal and differentiation in embryonic stem cells and may be associated with initiation, promotion, and progression in cancer. POU5F1 is a transcription factor and master regulator of cell pluripotency with indispensable roles in early embryo development and cell lineage specification. The role of embryonic POU5F1 in blastocyst formation and cell lineage specification differs between mammalian species. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to the C-terminus of the Human Oct4. |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
SCI 文献
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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