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Anti-OCLN Antibody (1S954) 是一种 Rabbit 抗体,靶向 OCLN。Anti-OCLN Antibody (1S954) 可用于 WB,IP,FCM。
Anti-OCLN Antibody (1S954) 是一种 Rabbit 抗体,靶向 OCLN。Anti-OCLN Antibody (1S954) 可用于 WB,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-OCLN Antibody (1S954) is a Rabbit antibody targeting OCLN. Anti-OCLN Antibody (1S954) can be used in WB,IP,FCM. |
别名 | Tight junction protein occludin, Occludin1, Occludin, Occludin-1, Occludin, MGC34277, FLJ94056, FLJ77961, FLJ18079, FLJ08163, BLCPMG |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Flow cytometric analysis of 293 cells with Occludin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. 2. All lanes use the Antibody at 1:1000 dilution for 1 hour at room temperature. 3. All lanes use the Antibody at 1:1000 dilution for 1 hour at room temperature. 4. All lanes use the Antibody at 1:1000 dilution for 1 hour at room temperature. |
应用 | |
推荐剂量 | WB: 1:1000; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by poly-unsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 59 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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