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Anti-NUF2 Antibody (5K725)
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产品编号 TMAC-02901
别名 NUF2R, NUF 2, Kinetochore protein Nuf2, hsNuf2, hNuf2R, hNuf2, Cell division cycle-associated protein 1, CDCA1
Anti-NUF2 Antibody (5K725) 是一种 Rabbit 抗体，靶向 NUF2。Anti-NUF2 Antibody (5K725) 可用于 WB,FCM。
Anti-NUF2 Antibody (5K725)
Anti-NUF2 Antibody (5K725)
还可以产品编号 TMAC-02901 别名 NUF2R, NUF 2, Kinetochore protein Nuf2, hsNuf2, hNuf2R, hNuf2, Cell division cycle-associated protein 1, CDCA1
Anti-NUF2 Antibody (5K725) 是一种 Rabbit 抗体，靶向 NUF2。Anti-NUF2 Antibody (5K725) 可用于 WB,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-NUF2 Antibody (5K725) is a Rabbit antibody targeting NUF2. Anti-NUF2 Antibody (5K725) can be used in WB,FCM. |
| 别名 | NUF2R, NUF 2, Kinetochore protein Nuf2, hsNuf2, hNuf2R, hNuf2, Cell division cycle-associated protein 1, CDCA1 |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of Nuf2 on different cell lysates using anti-Nuf2 at 1/1,000 dilution. Positive control: Line 1: K562, Line 2: PC-3M, Line 3: THP-1. 2. Flow cytometric analysis of A431 cells with Nuf2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: human Nuf2 aa 1-200 |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 54 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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