Anti-NRN1 Polyclonal Antibody is a Rabbit antibody targeting NRN1. Anti-NRN1 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit)

1. Sample: Brain (Mouse) lysate at 30 μg;
Primary: Anti-Neuritin (TMAB-01271) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size:16 kDa
Observed band size: 20 kDa
2. Blank control: U251. Primary Antibody (green line): Rabbit Anti-Neuritin antibody (TMAB-01271)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human Neuritin

Human