Anti-NR1I2 Polyclonal Antibody is a Rabbit antibody targeting NR1I2. Anti-NR1I2 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Pig,Cow,Horse)

1. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-PXR (TMAB-01265) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
2. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-PXR antibody (TMAB-01265)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 2ug/Test

Polyclonal

KLH conjugated synthetic peptide: human PXR

Human

Orphan Nuclear Receptors,Transcription Factors,Drug metabolism,Cancer,Hormones,Drug metabolism