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顶部生物活性储存和溶解信息计算器

Anti-nNOS Antibody (1J290)

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产品编号 TMAC-02869
别名 Peptidyl-cysteine S-nitrosylase NOS1, NOS1, NOS type I, nNOS), N-NOS, Nitric oxide synthase, brain, Neuronal NOS(N-NOS, neuronal Nitric Oxide Synthase, NC-NOS, EC:1.14.13.39, Constitutive NOS, bNOS

Anti-nNOS Antibody (1J290) 是一种 Rabbit 抗体，靶向 nNOS。Anti-nNOS Antibody (1J290) 可用于 FCM,ICC/IF,IHC,IP,WB。

Anti-nNOS Antibody (1J290)

Anti-nNOS Antibody (1J290)

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产品编号 TMAC-02869 别名 Peptidyl-cysteine S-nitrosylase NOS1, NOS1, NOS type I, nNOS), N-NOS, Nitric oxide synthase, brain, Neuronal NOS(N-NOS, neuronal Nitric Oxide Synthase, NC-NOS, EC:1.14.13.39, Constitutive NOS, bNOS

Anti-nNOS Antibody (1J290) 是一种 Rabbit 抗体，靶向 nNOS。Anti-nNOS Antibody (1J290) 可用于 FCM,ICC/IF,IHC,IP,WB。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-nNOS Antibody (1J290) is a Rabbit antibody targeting nNOS. Anti-nNOS Antibody (1J290) can be used in FCM,ICC/IF,IHC,IP,WB.
别名Peptidyl-cysteine S-nitrosylase NOS1, NOS1, NOS type I, nNOS), N-NOS, Nitric oxide synthase, brain, Neuronal NOS(N-NOS, neuronal Nitric Oxide Synthase, NC-NOS, EC:1.14.13.39, Constitutive NOS, bNOS
Ig Type
IgG
克隆号
1J290
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of nNOS on mouse heart lysates using anti-nNOS antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-nNOS antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-nNOS antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-nNOS antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-nNOS antibody. Counter stained with hematoxylin.
6. ICC staining nNOS in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining nNOS in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining nNOS in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of PC-12 cells with nNOS antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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应用FCMICC/IFIHCIPWB
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P29475
化学信息
分子量Theoretical: 161 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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