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Anti-NKX2-1 Antibody (6Z966)
还可以产品编号 TMAH-00818
别名 TTF-1, TTF1, TITF1, Thyroid-specific enhancer-binding protein, Thyroid transcription factor 1, Thyroid nuclear factor 1, T/EBP, NKX2A, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A
Anti-NKX2-1 Antibody (6Z966) 是一种抗体，靶向 NKX2-1。Anti-NKX2-1 Antibody (6Z966) 可用于 ELISA, WB, IHC, FC。
Anti-NKX2-1 Antibody (6Z966)
Anti-NKX2-1 Antibody (6Z966)
还可以产品编号 TMAH-00818 别名 TTF-1, TTF1, TITF1, Thyroid-specific enhancer-binding protein, Thyroid transcription factor 1, Thyroid nuclear factor 1, T/EBP, NKX2A, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A
Anti-NKX2-1 Antibody (6Z966) 是一种抗体，靶向 NKX2-1。Anti-NKX2-1 Antibody (6Z966) 可用于 ELISA, WB, IHC, FC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,315 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-NKX2-1 Antibody (6Z966) is an antibody targeting NKX2-1. Anti-NKX2-1 Antibody (6Z966) can be used in ELISA, WB, IHC, FC. |
| 别名 | TTF-1, TTF1, TITF1, Thyroid-specific enhancer-binding protein, Thyroid transcription factor 1, Thyroid nuclear factor 1, T/EBP, NKX2A, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A |
| Ig Type | Rabbit IgG |
| 克隆号 | 6Z966 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: HepG2 whole cell lysate, U251 whole cell lysate, U87 whole cell lysate -All lanes: TTF1 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 39 kDa -Observed band size: 39 kDa 2. IHC image of TMAH-00818 diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00818 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 4. Overlay histogram showing A549 cells stained with TMAH-00818 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | ELISA, WB, IHC, FC |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200; FC:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1. ... |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human TTF1 |
| 抗原种属 | Human |
| 基因ID | 7080 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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