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Anti-NF-kB p65 Antibody (6C69)
还可以产品编号 TMAY-02214
Anti-NF-kB p65 Antibody (6C69) 是一种 Mouse 抗体，靶向 NF-kB p65。Anti-NF-kB p65 Antibody (6C69) 可用于 ELISA,FCM,ICC/IF。
Anti-NF-kB p65 Antibody (6C69)
Anti-NF-kB p65 Antibody (6C69)
还可以产品编号 TMAY-02214
Anti-NF-kB p65 Antibody (6C69) 是一种 Mouse 抗体，靶向 NF-kB p65。Anti-NF-kB p65 Antibody (6C69) 可用于 ELISA,FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-NF-kB p65 Antibody (6C69) is a Mouse antibody targeting NF-kB p65. Anti-NF-kB p65 Antibody (6C69) can be used in ELISA,FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG2a |
| 克隆号 | 6C69 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of human RELA (NF-KB p65) in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-human RELA monoclonal antibody (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Purified anti-Human RelA/NFkB p65 on Jurkat cells (Left panel) and HeLa cells (Right panel). |
| 应用 | ELISA,FCM,ICC/IF |
| 推荐剂量 | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RELA / Transcription factor p65 (rh RELA / Transcription factor p65; TMPY-02387; Q04206-1; Met 1-Tyr 306). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | RELA (v-rel reticuloendotheliosis viral oncogene homolog A), also known as Nuclear factor NF-kappa-B p65 subunit, or Transcription factor p65, is a transcription factor expressed in growth plate chondrocytes where it facilitates chondrogenesis. The v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA) gene encodes the major component of the NF-?B complex. NF-kappaB is a generic name for an evolutionarily conserved transcription-factor system that contributes to the mounting of an effective immune response but is also involved in the regulation of cell proliferation, development, and apoptosis. The implication of NF-kappaB in central biological processes and its extraordinary connectivity to other signaling pathways raise a need for highly controlled regulation of NF-kappaB activity at several levels. The mammalian Rel/NF-kappaB family of transcription factors, including RelA, c-Rel, RelB, NF-kappaB1 (p50 and its precursor p105), and NF-kappaB2 (p52 and its precursor p100), plays a central role in the immune system by regulating several processes ranging from the development and survival of lymphocytes and lymphoid organs to the control of immune responses and malignant transformation. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human RELA / Transcription factor p65 protein (TMPY-02387) |
| 抗原种属 | Human |
| 研究领域 | Apoptosis Transcription Factors and Regulators |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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