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Anti-NeuN Antibody (8Z879)
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产品编号 TMAC-02782
别名 RNA binding protein fox-1 homolog 3, RFOX3, RBFOX3, Neuronal nuclei antigen(NeuN antigen), HRNBP3, FOX-3, FOX3, Fox-1 homolog C
Anti-NeuN Antibody (8Z879) 是一种 Rabbit 抗体，靶向 NeuN。Anti-NeuN Antibody (8Z879) 可用于 WB,ICC/IF,IHC,FCM。
Anti-NeuN Antibody (8Z879)
Anti-NeuN Antibody (8Z879)
还可以产品编号 TMAC-02782 别名 RNA binding protein fox-1 homolog 3, RFOX3, RBFOX3, Neuronal nuclei antigen(NeuN antigen), HRNBP3, FOX-3, FOX3, Fox-1 homolog C
Anti-NeuN Antibody (8Z879) 是一种 Rabbit 抗体，靶向 NeuN。Anti-NeuN Antibody (8Z879) 可用于 WB,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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抗原信息
化学信息
| 产品描述 | Anti-NeuN Antibody (8Z879) is a Rabbit antibody targeting NeuN. Anti-NeuN Antibody (8Z879) can be used in WB,ICC/IF,IHC,FCM. |
| 别名 | RNA binding protein fox-1 homolog 3, RFOX3, RBFOX3, Neuronal nuclei antigen(NeuN antigen), HRNBP3, FOX-3, FOX3, Fox-1 homolog C |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of NeuN on human brain lysates using anti-NeuN antibody at 1/1,000 dilution. (NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa). 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuN antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-NeuN antibody. Counter stained with hematoxylin. 4. ICC staining NeuN in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of SH-SY-5Y cells with NeuN antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:200-500; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 34 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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