Anti-NANOG Polyclonal Antibody is a Rabbit antibody targeting NANOG. Anti-NANOG Polyclonal Antibody can be used in IHC-P,FCM.

IgG

Human,Mouse (predicted:Rat)

1. Tissue/cell: Mouse tooth germ tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated (TMAB-01201) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti-Nanog antibody (TMAB-01201)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): f (ab/)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.

IHC-P: 1:100-500; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: mouse Nanog

Mouse

Cell differentiation,Surface molecules,SMADs,Transcription Factors,Intracellular