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Anti-NAA10/ARD1 Antibody (6X451)
还可以产品编号 TMAY-02715
Anti-NAA10/ARD1 Antibody (6X451) 是一种 Mouse 抗体，靶向 NAA10/ARD1。Anti-NAA10/ARD1 Antibody (6X451) 可用于 FCM,ICC/IF。
Anti-NAA10/ARD1 Antibody (6X451)
Anti-NAA10/ARD1 Antibody (6X451)
还可以产品编号 TMAY-02715
Anti-NAA10/ARD1 Antibody (6X451) 是一种 Mouse 抗体，靶向 NAA10/ARD1。Anti-NAA10/ARD1 Antibody (6X451) 可用于 FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-NAA10/ARD1 Antibody (6X451) is a Mouse antibody targeting NAA10/ARD1. Anti-NAA10/ARD1 Antibody (6X451) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG2a |
| 克隆号 | 6X451 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human ARD1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human ARD1A monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus. 2. Flow cytometric analysis of Human ARD1A expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human ARD1A, then a PE-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ARD1 (rh ARD1; TMPY-03339; P41227; Met1-Ser235). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | ARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating-type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human ARD1 protein (TMPY-03339) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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