Anti-MRC1 Antibody (6P560) is a Rabbit antibody targeting MRC1. Anti-MRC1 Antibody (6P560) can be used in WB,ICC/IF,FCM.

IgG

6P560

Human

1. The Molt-4 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-MRC1 antibody (TMAB-01168): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS.
2. 4% Paraformaldehyde-fixed Molt-4 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (MRC1) monoclonal Antibody, unconjugated (TMAB-01168) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green ) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.
3. 25 μg total protein per Lane of various lysates probed with MRC1 monoclonal antibody, unconjugated (TMAB-01168) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

WB: 1:500-2000; ICC/IF: 1:100; FCM: 1ug/Test

Monoclonal

A synthesized peptide: human MRC1

Human

Receptors,Macrophage / Inflamm.