Anti-MERTK/Mer Antibody (5M877) is a Mouse antibody targeting MERTK/Mer. Anti-MERTK/Mer Antibody (5M877) can be used in ELISA, WB, IF, FCM.

IgG2b

5M877

Human

1. Western Blot
-Positive WB detected in: 1-4 lanes: 293T whole cell lysate transfected with MERTK at 10ug, 5ug, 2.5ug, 1.25ug
-All lanes: MERTK antibody at 1:2000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 150-250 KDa
-Observed band size: 150-250 KDa
-Exposure time：5S
2. Western Blot
-Positive WB detected in: 1-4 lanes: Recombinant MERTK protein at 20ng, 10ng, 5ng, 2.5ng
-All lanes: MERTK antibody at 1:2000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 120 KDa
-Observed band size: 120-150 KDa
-Exposure time：5S
3. Western Blot
-Positive WB detected in: 293 whole cell lysate, SY5Y whole cell lysate, K562 whole cell lysate
-All lanes: MERTK antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 110 kDa
-Observed band size: 130 KDa
-Exposure time：5min
4. Immunofluorescence staining of 293T transfected cells with MERTK TMAH-00745 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
5. Immunofluorescence staining of HepG2 cells with MERTK TMAH-00745 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
6. Overlay Peak curve showing 293T transfected cells stained with TMAH-00745 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Monoclonal

Unconjugated

Recombinant Protein: MERTK Protein (21-505AA)

Human

Signal Transduction