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Anti-MEKK2 Antibody (5G332)
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产品编号 TMAC-02556
别名 Mitogen-activated protein kinase kinase kinase 2, Mitogen activated protein kinase kinase kinase 2, MEKK2b, MEKK2, MEK kinase 2, MAPK/ERK kinase kinase 2, Map3k2, M3K2_HUMAN
Anti-MEKK2 Antibody (5G332) 是一种 Rabbit 抗体，靶向 MEKK2。Anti-MEKK2 Antibody (5G332) 可用于 WB,ICC,IHC,IP,FCM。
Anti-MEKK2 Antibody (5G332)
Anti-MEKK2 Antibody (5G332)
还可以产品编号 TMAC-02556 别名 Mitogen-activated protein kinase kinase kinase 2, Mitogen activated protein kinase kinase kinase 2, MEKK2b, MEKK2, MEK kinase 2, MAPK/ERK kinase kinase 2, Map3k2, M3K2_HUMAN
Anti-MEKK2 Antibody (5G332) 是一种 Rabbit 抗体，靶向 MEKK2。Anti-MEKK2 Antibody (5G332) 可用于 WB,ICC,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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偶联与修饰
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| 产品描述 | Anti-MEKK2 Antibody (5G332) is a Rabbit antibody targeting MEKK2. Anti-MEKK2 Antibody (5G332) can be used in WB,ICC,IHC,IP,FCM. |
| 别名 | Mitogen-activated protein kinase kinase kinase 2, Mitogen activated protein kinase kinase kinase 2, MEKK2b, MEKK2, MEK kinase 2, MAPK/ERK kinase kinase 2, Map3k2, M3K2_HUMAN |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of MEKK2 on different lysates using anti-MEKK2 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: Rat brain, Lane 3: SW480. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEKK2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MEKK2 antibody. Counter stained with hematoxylin. 4. ICC staining MEKK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining MEKK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining MEKK2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of HepG2 cells with MEKK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK 1 activates both MEK-4 and MEK-3/MEK-6 pathways. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 70 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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