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顶部生物活性储存和溶解信息计算器

Anti-MCP-3/CCL7 Antibody (4B549)

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Anti-MCP-3/CCL7 Antibody (4B549)
产品编号 TMAY-02144
别名 Scya7, MCP-3, mcp3, marc, fic, chemokine (C-C motif) ligand 7

Anti-MCP-3/CCL7 Antibody (4B549) 是一种 Mouse 抗体，靶向 MCP-3/CCL7。Anti-MCP-3/CCL7 Antibody (4B549) 可用于 ELISA,FCM,ICC/IF。

Anti-MCP-3/CCL7 Antibody (4B549)
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Anti-MCP-3/CCL7 Antibody (4B549)

Rating icon 还可以
Anti-MCP-3/CCL7 Antibody (4B549)
产品编号 TMAY-02144 别名 Scya7, MCP-3, mcp3, marc, fic, chemokine (C-C motif) ligand 7

Anti-MCP-3/CCL7 Antibody (4B549) 是一种 Mouse 抗体，靶向 MCP-3/CCL7。Anti-MCP-3/CCL7 Antibody (4B549) 可用于 ELISA,FCM,ICC/IF。

规格价格库存数量
100 μL
¥ 1,694
5日内发货
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产品介绍

生物活性
产品描述
Anti-MCP-3/CCL7 Antibody (4B549) is a Mouse antibody targeting MCP-3/CCL7. Anti-MCP-3/CCL7 Antibody (4B549) can be used in ELISA,FCM,ICC/IF.
别名Scya7, MCP-3, mcp3, marc, fic, chemokine (C-C motif) ligand 7
Ig Type
Mouse IgG1
克隆号
4B549
交叉反应
Human
验证活性
1. Immunofluorescence staining of Human CCL7 in SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CCL7 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.
2. Flow cytometric analysis of Human CCL7 expression on THP-1 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CCL7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
应用
ELISA,FCM,ICC/IF
推荐剂量
ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100
抗体种类
Monoclonal
宿主来源Mouse
构建方式This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CCL7 / MCP3 (rh CCL7 / MCP3; NP_006264.2Gln 24-Leu 99). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 7(CCL7), also known as MCP-3, is a isform of the C-C chemokine subfamily of the chemokine family which is produced by certain tumor cells and by macrophages. It also own two adjacent N-terminal cysteine residues. Chemokine ligand 7(CCL7) spacifically attracts monocytes, and regulates macrophage function.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
PE
可提供抗体种类
2
抗原信息
免疫原
Recombinant Protein: Human CCL7 / MCP3 protein
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

计算器

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关键词

MCP 3

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