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Anti-MCM3 Antibody (3T338)
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产品编号 TMAC-02498
别名 RLFB, RLF subunit beta, RLF beta subunit, Replication licensing factor beta subunit, P1-MCM3, P102 protein, p102, P1.h, P1 Protein, P1 MCM3, P1 h, Minichromosome maintenance protein 3, Minichromosome maintenance deficient 3, Minichromosome maintenance complex component 3, MGC1157, MCM3_HUMAN, MCM3 minichromosome maintenance deficient 3, mcm3, MCM 3, Human cervical cancer proto oncogene 5, hRlf beta subunit, HCC5, HCC 5, DNA replication licensing factor mcm3, DNA replication factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, DNA polymerase alpha holoenzyme associated protein P1, DNA polymerase alpha holoenzyme associated P1, Cervical cancer proto oncogene 5
Anti-MCM3 Antibody (3T338) 是一种 Rabbit 抗体，靶向 MCM3。Anti-MCM3 Antibody (3T338) 可用于 WB,ICC,IF,IHC,FCM。
Anti-MCM3 Antibody (3T338)
Anti-MCM3 Antibody (3T338)
还可以产品编号 TMAC-02498 别名 RLFB, RLF subunit beta, RLF beta subunit, Replication licensing factor beta subunit, P1-MCM3, P102 protein, p102, P1.h, P1 Protein, P1 MCM3, P1 h, Minichromosome maintenance protein 3, Minichromosome maintenance deficient 3, Minichromosome maintenance complex component 3, MGC1157, MCM3_HUMAN, MCM3 minichromosome maintenance deficient 3, mcm3, MCM 3, Human cervical cancer proto oncogene 5, hRlf beta subunit, HCC5, HCC 5, DNA replication licensing factor mcm3, DNA replication factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, DNA polymerase alpha holoenzyme associated protein P1, DNA polymerase alpha holoenzyme associated P1, Cervical cancer proto oncogene 5
Anti-MCM3 Antibody (3T338) 是一种 Rabbit 抗体，靶向 MCM3。Anti-MCM3 Antibody (3T338) 可用于 WB,ICC,IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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| 产品描述 | Anti-MCM3 Antibody (3T338) is a Rabbit antibody targeting MCM3. Anti-MCM3 Antibody (3T338) can be used in WB,ICC,IF,IHC,FCM. |
| 别名 | RLFB, RLF subunit beta, RLF beta subunit, Replication licensing factor beta subunit, P1-MCM3, P102 protein, p102, P1.h, P1 Protein, P1 MCM3, P1 h, Minichromosome maintenance protein 3, Minichromosome maintenance deficient 3, Minichromosome maintenance complex component 3, MGC1157, MCM3_HUMAN, MCM3 minichromosome maintenance deficient 3, mcm3, MCM 3, Human cervical cancer proto oncogene 5, hRlf beta subunit, HCC5, HCC 5, DNA replication licensing factor mcm3, DNA replication factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, DNA polymerase alpha holoenzyme associated protein P1, DNA polymerase alpha holoenzyme associated P1, Cervical cancer proto oncogene 5 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of MCM3 on mouse thymus tissue (1), NIH-3T3 cell (2) and Hela (3)cell lysates using anti-MCM3 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-MCM3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM3 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-MCM3 antibody. Counter stained with hematoxylin. 6. ICC staining MCM3 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining MCM3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining MCM3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of K562 cells with MCM3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2?C6. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-γ-induced Stat1α transcription activation. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 102 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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