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Anti-MBTPS1 Antibody (3A692)

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Anti-MBTPS1 Antibody (3A692)
产品编号 TMAH-00741

Anti-MBTPS1 Antibody (3A692) 是一种 Mouse 抗体,靶向 MBTPS1。Anti-MBTPS1 Antibody (3A692) 可用于 ELISA, WB, FCM。

Anti-MBTPS1 Antibody (3A692)

Anti-MBTPS1 Antibody (3A692)

Rating icon 还可以
Anti-MBTPS1 Antibody (3A692)
产品编号 TMAH-00741

Anti-MBTPS1 Antibody (3A692) 是一种 Mouse 抗体,靶向 MBTPS1。Anti-MBTPS1 Antibody (3A692) 可用于 ELISA, WB, FCM。

规格价格库存数量
50 μL
¥ 1,310
5日内发货
100 μL
¥ 2,180
5日内发货
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产品介绍

生物活性
产品描述
Anti-MBTPS1 Antibody (3A692) is a Mouse antibody targeting MBTPS1. Anti-MBTPS1 Antibody (3A692) can be used in ELISA, WB, FCM.
Ig Type
IgG1
克隆号
3A692
交叉反应
Human
验证活性
1. Western Blot
-Positive WB detected in: U251 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate
-All lanes: MBTPS1 antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 117 kDa
-Observed band size: 117 KDa
-Exposure time:5min
2. Overlay Peak curve showing Hela cells stained with TMAH-00741 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
应用
抗体种类
Monoclonal
宿主来源Mouse
亚细胞定位Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein.
构建方式Hybridoma Monoclonal Antibody
纯化方式Protein G purified
性状Liquid
缓冲液Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
纯度>95%
研究背景Serine protease that cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4: known substrates are SREBF1/SREBP1, SREBF2/SREBP2, BDNF, GNPTAB, ATF6 and ATF6B. Cleaves substrates after Arg-Ser-Val-Leu (SREBP2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. Catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2. Also mediates the first step in the proteolytic activation of the cyclic AMP-dependent transcription factor ATF-6 (ATF6 and ATF6B). Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes. Involved in the regulation of M6P-dependent Golgi-to-lysosome trafficking of lysosomal enzymes. It is required for the activation of CREB3L2/BBF2H7, a transcriptional activator of MIA3/TANGO and other genes controlling mega vesicle formation. Therefore, it plays a key role in the regulation of mega vesicle-mediated collagen trafficking.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human Membrane-bound transcription factor site-1 protease Protein (187-400aa)
抗原种属
Human
基因ID
Uniprot ID
研究领域
Epigenetics
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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