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Anti-MBTPS1 Antibody (3A692) 是一种 Mouse 抗体,靶向 MBTPS1。Anti-MBTPS1 Antibody (3A692) 可用于 ELISA, WB, FCM。
Anti-MBTPS1 Antibody (3A692) 是一种 Mouse 抗体,靶向 MBTPS1。Anti-MBTPS1 Antibody (3A692) 可用于 ELISA, WB, FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,310 | 5日内发货 | |
100 μL | ¥ 2,180 | 5日内发货 |
产品描述 | Anti-MBTPS1 Antibody (3A692) is a Mouse antibody targeting MBTPS1. Anti-MBTPS1 Antibody (3A692) can be used in ELISA, WB, FCM. |
Ig Type | IgG1 |
克隆号 | 3A692 |
交叉反应 | Human |
验证活性 | 1. Western Blot -Positive WB detected in: U251 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate -All lanes: MBTPS1 antibody at 1:1000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 117 kDa -Observed band size: 117 KDa -Exposure time:5min 2. Overlay Peak curve showing Hela cells stained with TMAH-00741 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
应用 | |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
亚细胞定位 | Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | Protein G purified |
性状 | Liquid |
缓冲液 | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
纯度 | >95% |
研究背景 | Serine protease that cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4: known substrates are SREBF1/SREBP1, SREBF2/SREBP2, BDNF, GNPTAB, ATF6 and ATF6B. Cleaves substrates after Arg-Ser-Val-Leu (SREBP2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. Catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2. Also mediates the first step in the proteolytic activation of the cyclic AMP-dependent transcription factor ATF-6 (ATF6 and ATF6B). Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes. Involved in the regulation of M6P-dependent Golgi-to-lysosome trafficking of lysosomal enzymes. It is required for the activation of CREB3L2/BBF2H7, a transcriptional activator of MIA3/TANGO and other genes controlling mega vesicle formation. Therefore, it plays a key role in the regulation of mega vesicle-mediated collagen trafficking. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein: Human Membrane-bound transcription factor site-1 protease Protein (187-400aa) |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Epigenetics |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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