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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-MBP tag Polyclonal Antibody

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货号 TMAB-08651

Anti-MBP tag Polyclonal Antibody 是一种 Rabbit 抗体，靶向 MBP tag。Anti-MBP tag Polyclonal Antibody 可用于 WB,ELISA。

Anti-MBP tag Polyclonal Antibody

Anti-MBP tag Polyclonal Antibody

一键复制产品信息
Rating icon 还可以
货号 TMAB-08651

Anti-MBP tag Polyclonal Antibody 是一种 Rabbit 抗体，靶向 MBP tag。Anti-MBP tag Polyclonal Antibody 可用于 WB,ELISA。

规格价格库存数量
100 μL
¥ 970
5日内发货
500 μL
¥ 3,995
5日内发货
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产品介绍

生物活性
产品描述
Anti-MBP tag Polyclonal Antibody is a Rabbit antibody targeting MBP tag. Anti-MBP tag Polyclonal Antibody can be used in WB,ELISA.
Ig Type
IgG
反应种属
Species independent
应用WBELISA
推荐剂量
WB: 1:500-2000; ELISA: 1:1000-5000
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Periplasm.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.
抗原信息
免疫原
KLH conjugated synthetic peptide: E. coli Maltose Binding Protein
基因名称
malE
基因ID
1039564
蛋白名称
Maltose/maltodextrin-binding periplasmic protein
功能
Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody.
化学信息
分子量Theoretical: 44 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | -20°C for 1 year

计算器

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