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Anti-MBD3 Antibody (8O286)
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产品编号 TMAC-02484
别名 Methyl-CpG-binding protein MBD3, Methyl-CpG-binding domain protein 3, Methyl CpG binding protein MBD3, Methyl CpG binding domain protein 3, MBD3_HUMAN, MBD3: methyl CpG binding domain protein 3, Mbd3, MBD 3, AU019209, AI181826
Anti-MBD3 Antibody (8O286) 是一种 Rabbit 抗体，靶向 MBD3。Anti-MBD3 Antibody (8O286) 可用于 WB,ICC,IF,IHC,FCM,IP。
Anti-MBD3 Antibody (8O286)
Anti-MBD3 Antibody (8O286)
还可以产品编号 TMAC-02484 别名 Methyl-CpG-binding protein MBD3, Methyl-CpG-binding domain protein 3, Methyl CpG binding protein MBD3, Methyl CpG binding domain protein 3, MBD3_HUMAN, MBD3: methyl CpG binding domain protein 3, Mbd3, MBD 3, AU019209, AI181826
Anti-MBD3 Antibody (8O286) 是一种 Rabbit 抗体，靶向 MBD3。Anti-MBD3 Antibody (8O286) 可用于 WB,ICC,IF,IHC,FCM,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-MBD3 Antibody (8O286) is a Rabbit antibody targeting MBD3. Anti-MBD3 Antibody (8O286) can be used in WB,ICC,IF,IHC,FCM,IP. |
| 别名 | Methyl-CpG-binding protein MBD3, Methyl-CpG-binding domain protein 3, Methyl CpG binding protein MBD3, Methyl CpG binding domain protein 3, MBD3_HUMAN, MBD3: methyl CpG binding domain protein 3, Mbd3, MBD 3, AU019209, AI181826 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of MBD3 on U937 cell lysate using anti-MBD3 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-MBD3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MBD3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MBD3 antibody. Counter stained with hematoxylin. 5. ICC staining MBD3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining MBD3 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining MBD3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of SH-SY-5Y cells with MBD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Methylation of DNA contributes to the regulation of gene transcription in both mammalian and invertebrate systems. DNA methylation predominates on cytosine residues that are present in dinucleotide motifs consisting of a 5?? cytosine followed by guanosine (CpG), and it requires the enzymatic activity of DNA methyltransferase, which results in transcriptional repression of the methylated gene. Several proteins have been identified that associate with the methyl-CpG sites, and they include methyl-CpG binding protein-1 (MBD1), MBD2, MBD3, MBD4 and MeCP2. Expression of the MBD proteins is highest in somatic tissues. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 33 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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