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Anti-Marapsin Antibody (8L443)

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Anti-Marapsin Antibody (8L443)
产品编号 TMAY-02243
别名 UNQ1884/PRO4327, protease, serine 27, MPN, CAPH2

Anti-Marapsin Antibody (8L443) 是一种 Rabbit 抗体,靶向 Marapsin。Anti-Marapsin Antibody (8L443) 可用于 WB,IP。

Anti-Marapsin Antibody (8L443)
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Anti-Marapsin Antibody (8L443)

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Anti-Marapsin Antibody (8L443)
产品编号 TMAY-02243 别名 UNQ1884/PRO4327, protease, serine 27, MPN, CAPH2

Anti-Marapsin Antibody (8L443) 是一种 Rabbit 抗体,靶向 Marapsin。Anti-Marapsin Antibody (8L443) 可用于 WB,IP。

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100 μL
¥ 2,289
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产品介绍

生物活性
产品描述
Anti-Marapsin Antibody (8L443) is a Rabbit antibody targeting Marapsin. Anti-Marapsin Antibody (8L443) can be used in WB,IP.
别名UNQ1884/PRO4327, protease, serine 27, MPN, CAPH2
Ig Type
Rabbit IgG
克隆号
8L443
交叉反应
Human
验证活性
1. Anti-PRSS27 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: K562 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:32 kDa.
-Observed band size:32 kDa.
2. PRSS27 was immunoprecipitated using:
-Lane A:0.5 mg K562 Whole Cell Lysate.
-2 µL anti-PRSS27 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-PRSS27 rabbit monoclonal antibody, at 1:200 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 32 kDa.
-Observed band size: 32 kDa
应用
WB,IP
推荐剂量
WB: 1:500-1:2000; IP: 4-6 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human Pancreasin / Marapsin / PRSS27 (rh Pancreasin / Marapsin / PRSS27; TMPY-02117; Q9BQR3; Met1-Lys290).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景The name "Pancreasin" because it is transcribed strongly in the pancreas. This secreted, tryptic serine protease, also known as Marapsin or PRSS27 (Protease, serine, 27), is a member of the peptidase S1 family. Pancreasin is inhibited by benzamidine and leupeptin but resists several classic inhibitors of trypsin. Marapsin was constitutively expressed in nonkeratinizing stratified squamous epithelia of human esophagus, tonsil, cervix, larynx, and cornea. In fact, marapsin was the second most strongly up-regulated protease in psoriatic lesions, where expression was localized to the upper region of the hyperplastic epidermis. Similarly, in the hyperproliferative epithelium of regenerating murine skin wounds, marapsin localized to the suprabasal layers, where keratinocytes undergo squamous differentiation. Marapsin's restricted expression, localization, and cytokine-inducible expression suggest a role in the terminal differentiation of keratinocytes in hyperproliferating squamous epithelia.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human Pancreasin / Marapsin / PRSS27 protein (TMPY-02117)
抗原种属
Human
研究领域
Serine Proteases and Regulators
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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