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Anti-MAP2K3 Antibody (7F635)
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产品编号 TMAC-02546
别名 zMKK 3, SKK2, Protein kinase mitogen activated kinase 3, PRKMK3, PRKMK 3, MPK 3, MP2K6, MP2K3, mMKK3b, mMKK 3b, MKK3, MKK 3, Mitogen activated protein kinase kinase 3, MEK3, MEK 3, MAPKK3, MAPKK 3, MAPK kinase 3, MAPK ERK kinase 3, MAP2K 3, MAP Kinase Kinase 3, MAP kinase kinase 3, ERK kinase 3, Dual specificity mitogen activated protein kinase kinase 3
Anti-MAP2K3 Antibody (7F635) 是一种 Rabbit 抗体，靶向 MAP2K3。Anti-MAP2K3 Antibody (7F635) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-MAP2K3 Antibody (7F635)
Anti-MAP2K3 Antibody (7F635)
还可以产品编号 TMAC-02546 别名 zMKK 3, SKK2, Protein kinase mitogen activated kinase 3, PRKMK3, PRKMK 3, MPK 3, MP2K6, MP2K3, mMKK3b, mMKK 3b, MKK3, MKK 3, Mitogen activated protein kinase kinase 3, MEK3, MEK 3, MAPKK3, MAPKK 3, MAPK kinase 3, MAPK ERK kinase 3, MAP2K 3, MAP Kinase Kinase 3, MAP kinase kinase 3, ERK kinase 3, Dual specificity mitogen activated protein kinase kinase 3
Anti-MAP2K3 Antibody (7F635) 是一种 Rabbit 抗体，靶向 MAP2K3。Anti-MAP2K3 Antibody (7F635) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-MAP2K3 Antibody (7F635) is a Rabbit antibody targeting MAP2K3. Anti-MAP2K3 Antibody (7F635) can be used in WB,ICC/IF,IHC,IP,FCM. |
| 别名 | zMKK 3, SKK2, Protein kinase mitogen activated kinase 3, PRKMK3, PRKMK 3, MPK 3, MP2K6, MP2K3, mMKK3b, mMKK 3b, MKK3, MKK 3, Mitogen activated protein kinase kinase 3, MEK3, MEK 3, MAPKK3, MAPKK 3, MAPK kinase 3, MAPK ERK kinase 3, MAP2K 3, MAP Kinase Kinase 3, MAP kinase kinase 3, ERK kinase 3, Dual specificity mitogen activated protein kinase kinase 3 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of MEK3+MEK6 on different lysates using anti-MEK3+MEK6 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: Jurkat. 2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MEK3+MEK6 antibody. Counter stained with hematoxylin. 3. ICC staining MEK3+MEK6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining MEK3+MEK6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining MEK3+MEK6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela cells with MEK3+MEK6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 39/37 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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