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Anti-M13 Bacteriophage Antibody-FITC (4Z973)
还可以产品编号 TMAY-02172F
Anti-M13 Bacteriophage Antibody-FITC (4Z973) 是一种 FITC-conjugated Mouse 抗体，靶向 M13 Bacteriophage。Anti-M13 Bacteriophage Antibody-FITC (4Z973) 可用于 FCM。
Anti-M13 Bacteriophage Antibody-FITC (4Z973)
Anti-M13 Bacteriophage Antibody-FITC (4Z973)
还可以产品编号 TMAY-02172F
Anti-M13 Bacteriophage Antibody-FITC (4Z973) 是一种 FITC-conjugated Mouse 抗体，靶向 M13 Bacteriophage。Anti-M13 Bacteriophage Antibody-FITC (4Z973) 可用于 FCM。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-M13 Bacteriophage Antibody-FITC (4Z973) is a FITC-conjugated Mouse antibody targeting M13 Bacteriophage. Anti-M13 Bacteriophage Antibody-FITC (4Z973) can be used in FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 4Z973 |
| 交叉反应 | other |
| 验证活性 | U266 cells were incubated with M13 phage particles for 20 minutes, then detected with 10 μL phage M13-FITC Ab. U266 stained with a cell-line specific M13 phage antibody (filied profile). Compared to an irrelevant phage antibody (open profile). The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | FCM |
| 推荐剂量 | 10 μl/Test, 0.1 mg/ml |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This product is a recombinant monoclonal antibody expressed from HEK293 cells. Then conjugated with FITC under optimum conditions, the unreacted FITC was removed. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| 研究背景 | M13 is a filamentous bacteriophage composed of circular single-stranded DNA (ssDNA) which is 647 nucleotides long encapsulated in approximately 27 copies of the major coat protein P8 and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with filamentous phages is not lethal, however, the infection causes turbid plaques in E. coli. It is a non-lytic virus. However, a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology. The phage coat is primarily assembled from a 5 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome. For a wild type M13 particle, it takes about approximately 27 copies of p8 to make the coat about 9 nm long. The coat's dimensions are flexible though and the number of p8 copies adjusts to accommodate the size of the single-stranded genome it packages. The general stages to a viral life cycle are infection, replication of the viral genome, assembly of new viral particles, and then release of the progeny particles from the host. Filamentous phage uses a bacterial structure known as the F pilus to infect E. coli, with the M13 p3 tip contacting the TolA protein on the bacterial pilus. The phage genome is then transferred to the cytoplasm of the bacterial cell where resident proteins convert the single-stranded DNA genome to a double-stranded replicative form. |
| 偶联 | FITC |
| 其他可选修饰符 | Unconjugated/Biotin/HRP/PE |
| 可提供抗体种类 | 5 |
| 免疫原 | M13 Bacteriophage |
| 抗原种属 | other |
存储&运输
| 储存方式 | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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