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顶部生物活性储存和溶解信息计算器

Anti-LYVE1 Antibody (5P861)

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产品编号 TMAH-00716

别名 XLKD1, LYVE-1, Lymphatic vessel endothelial hyaluronic acid receptor 1, Hyaluronic acid receptor, HAR, Extracellular link domain-containing protein 1, CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1

Anti-LYVE1 Antibody (5P861) 是一种抗体，靶向 LYVE1。Anti-LYVE1 Antibody (5P861) 可用于 ELISA,FCM,IF。

Anti-LYVE1 Antibody (5P861)

Anti-LYVE1 Antibody (5P861)

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Rating icon 还可以
产品编号 TMAH-00716 别名 XLKD1, LYVE-1, Lymphatic vessel endothelial hyaluronic acid receptor 1, Hyaluronic acid receptor, HAR, Extracellular link domain-containing protein 1, CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1

Anti-LYVE1 Antibody (5P861) 是一种抗体，靶向 LYVE1。Anti-LYVE1 Antibody (5P861) 可用于 ELISA,FCM,IF。

规格价格库存数量
50 μL
¥ 1,315
5日内发货
100 μL
¥ 2,185
5日内发货
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产品介绍

生物活性
产品描述
Anti-LYVE1 Antibody (5P861) is an antibody targeting LYVE1. Anti-LYVE1 Antibody (5P861) can be used in ELISA,FCM,IF.
别名XLKD1, LYVE-1, Lymphatic vessel endothelial hyaluronic acid receptor 1, Hyaluronic acid receptor, HAR, Extracellular link domain-containing protein 1, CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1
Ig Type
Rabbit IgG
克隆号
5P861
交叉反应
Human
验证活性
1. Immunofluorescence staining of HepG2 with TMAH-00716 at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 505-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
2. Overlay Peak curve showing HepG2 cells surface stained with TMAH-00716 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
应用ELISAFCMIF
推荐剂量
IF:1:50-1:200; FCM:1:50-1:200.
抗体种类
Monoclonal
亚细胞定位Membrane; Single-pass type I membrane protein. Note=Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
组织特异性Mainly expressed in endothelial cells lining lymphatic vessels.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human LYVE1
抗原种属
Human
基因名称
LYVE1
基因ID
10894
Uniprot ID
Q9Y5Y7
研究领域
Cardiovascular
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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