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Anti-LYPD3 Antibody (5Y943)

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产品编号 TMAY-02083

Anti-LYPD3 Antibody (5Y943) 是一种 Rabbit 抗体,靶向 LYPD3。Anti-LYPD3 Antibody (5Y943) 可用于 ELISA,FCM,ICC/IF。

Anti-LYPD3 Antibody (5Y943)

Anti-LYPD3 Antibody (5Y943)

Rating icon 还可以
产品编号 TMAY-02083

Anti-LYPD3 Antibody (5Y943) 是一种 Rabbit 抗体,靶向 LYPD3。Anti-LYPD3 Antibody (5Y943) 可用于 ELISA,FCM,ICC/IF。

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产品介绍

生物活性
产品描述
Anti-LYPD3 Antibody (5Y943) is a Rabbit antibody targeting LYPD3. Anti-LYPD3 Antibody (5Y943) can be used in ELISA,FCM,ICC/IF.
Ig Type
Monoclonal Rabbit IgG
克隆号
5Y943
交叉反应
Human
验证活性
1. Immunofluorescence staining of Human LYPD3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human LYPD3 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.

2. Flow cytometric analysis of Human LYPD3 expression on PC-3 cells. Cells were stained with purified anti-Human LYPD3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

3. Anti-LYPD3 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: Hela Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:36 kDa.

-Observed band size:36 kDa
应用
ELISA,FCM,ICC/IF
推荐剂量
ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human C4.4A / LYPD3 (rh C4.4A / LYPD3; TMPY-01859; NP_055215.2; Met 1-His 286).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Ly6 / PLAUR domain-containing protein 3, also known as GPI-anchored metastasis-associated protein C4.4A homolog, Matrigel-induced gene C4 protein, MIG-C4, and LYPD3, is a cell membrane protein that contains two UPAR/Ly6 domains. Human LYPD3 contains two UPAR/Ly6 domains. LYPD3 is expressed in the placenta, skin, and urothelium. It is found in suprabasal keratinocytes of chronic wounds. Weak expression of LYPD3 is found in the esophagus and peripheral blood mononuclear cells. It is found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression of LYPD3 is found in the tumor component of some noninvasive superficial lesions and invasive and metastatic urothelial cancers. LYPD3 is up-regulated in migrating keratinocytes during epithelisation of incisional skin wounds. LYPD3 supports cell migration. It may be involved in urothelial cell-matrix interactions. It may also be involved in tumor progression
偶联与修饰
偶联
Unconjugated
其他可选修饰符
APC/PE
可提供抗体种类
3
抗原信息
免疫原
Recombinant Human C4.4A / LYPD3 protein (TMPY-01859)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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