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Anti-LOX Antibody (2A200) 是一种 Rabbit 抗体,靶向 LOX。Anti-LOX Antibody (2A200) 可用于 WB,ICC,IHC,FCM。
Anti-LOX Antibody (2A200) 是一种 Rabbit 抗体,靶向 LOX。Anti-LOX Antibody (2A200) 可用于 WB,ICC,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-LOX Antibody (2A200) is a Rabbit antibody targeting LOX. Anti-LOX Antibody (2A200) can be used in WB,ICC,IHC,FCM. |
别名 | Protein-lysine 6-oxidase, Lysyl oxidase, LOX |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat,zebrafish |
验证活性 | 1. Western blot analysis of LOX on different lysates using anti-LOX antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: Mouse brain, Lane 3: Human fetal brain. 2. Western blot analysis of LOX on Zebrafish tissue lysates using anti-LOX antibody at 1/1,000 dilution. 3. Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-LOX antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LOX antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOX antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-LOX antibody. Counter stained with hematoxylin. 7. ICC staining LOX in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining LOX in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining LOX in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of Hela cells with LOX antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-100; ICC: 1:100-500; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The lysyl oxidase family of extracellular proteins includes LOX and four LOX-like enzymes, which are responsible for the deamination of peptidyl lysine residues of collagens and elastin. They also catalyze inter- and intra-crosslinking reactions. Overexpression of LOX may cause severe fibrotic degeneration due to its high resistance to degradative enzymes. Procollagen C-proteinase activity processes LOX from a precursor protein to a mature form. Activation of LOX occurs in normal developing and adult skin, and alterations in LOX expression and activity are associated with skin aging and senescence. LOX is crucial for development of the cardiovascular and respiratory systems. In addition, LOX plays a role in cancer, wound healing, as well as cell motility, chemotaxis and differentiation. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 50 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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