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Anti-LIMS1 Antibody (3E803) 是一种 Mouse 抗体,靶向 LIMS1。Anti-LIMS1 Antibody (3E803) 可用于 WB,ICC,FCM。
Anti-LIMS1 Antibody (3E803) 是一种 Mouse 抗体,靶向 LIMS1。Anti-LIMS1 Antibody (3E803) 可用于 WB,ICC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,980 | 5日内发货 | |
100 μL | ¥ 2,990 | 5日内发货 |
产品描述 | Anti-LIMS1 Antibody (3E803) is a Mouse antibody targeting LIMS1. Anti-LIMS1 Antibody (3E803) can be used in WB,ICC,FCM. |
别名 | Pinch1, Particularly interesting new Cys-His protein 1 (PINCH-1), Lims1, LIM and senescent cell antigen-like-containing domain protein 1 |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of PINCH on human PINCH recombinant protein using anti-PINCH antibody at 1/1,000 dilution. 2. Western blot analysis of PINCH on different cell lysates using anti-PINCH antibody at 1/1,000 dilution. Positive control: Lane 1: A549, Lane 2: Jurkat, Lane 3: Hela. 3. ICC staining PINCH (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of Hela cells with PINCH antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). |
应用 | |
推荐剂量 | WB: 1:500-1000; ICC: 1:100-500; FCM: 1:100-200 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
研究背景 | Pinch, also designated particularly interesting new Cys-His protein or NY-REN-48, is a focal adhesion protein that is a component of the ILK-PINCH complex. This complex is a major part of the growth factor and integrin signaling pathway. Pinch is involved in cell differentiation, proliferation and survival by acting as an effector of integrin and growth factor signaling. It is a cytoplasmic protein expressed in most tissues and consists of five LIM domains, a nuclear localization signal and a nuclear export signal. The PINCH-1/ILK complex is regulated by a PINCH-1 related protein PINCH-2, which also forms a complex with ILK. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 37 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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