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Anti-LDLR Antibody (3N325)
还可以产品编号 TMAY-03221
Anti-LDLR Antibody (3N325) 是一种 Rabbit 抗体，靶向 LDLR。Anti-LDLR Antibody (3N325) 可用于 ELISA,FCM,ICC/IF。
Anti-LDLR Antibody (3N325)
Anti-LDLR Antibody (3N325)
还可以产品编号 TMAY-03221
Anti-LDLR Antibody (3N325) 是一种 Rabbit 抗体，靶向 LDLR。Anti-LDLR Antibody (3N325) 可用于 ELISA,FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-LDLR Antibody (3N325) is a Rabbit antibody targeting LDLR. Anti-LDLR Antibody (3N325) can be used in ELISA,FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 3N325 |
| 交叉反应 | Mouse |
| 验证活性 | 1. Immunofluorescence staining of Mouse LDLR in raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse LDLR monoclonal antibody (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-rabbit IgG secondary antibody(green), countstained with DAPI (blue). Positive staining was localized to plasma membrane. 2. Flow cytometric analysis of Mouse LDLR expression on Raw264.7. Cells were stained with purified anti-Mouse LDLR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | ELISA,FCM,ICC/IF |
| 推荐剂量 | ELISA: 1:5000-1:10000; ICC-IF: 1:50-1:1000; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LDLR / LDL Receptor (rM LDLR; TMPY-01014; Met 1-Arg 790; NP_034830.2). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | LDL Receptor, also known as LDLR, is a mosaic protein that belongs to the Low-density lipoprotein receptor gene family. The low-density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 840 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low-density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | PerCP/FITC/PE |
| 可提供抗体种类 | 4 |
| 免疫原 | Recombinant Mouse LDLR protein (TMPY-01014) |
| 抗原种属 | Mouse |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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