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Anti-KRT6A Antibody (4O729) 是一种抗体,靶向 KRT6A。Anti-KRT6A Antibody (4O729) 可用于 ELISA, IF, FC。
Anti-KRT6A Antibody (4O729) 是一种抗体,靶向 KRT6A。Anti-KRT6A Antibody (4O729) 可用于 ELISA, IF, FC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,310 | 5日内发货 | |
100 μL | ¥ 2,190 | 5日内发货 |
产品描述 | Anti-KRT6A Antibody (4O729) is an antibody targeting KRT6A. Anti-KRT6A Antibody (4O729) can be used in ELISA, IF, FC. |
别名 | Type-II keratin Kb6, type II cytoskeletal 6A, KRT 6A, Keratin-6A, Keratin, K6A KRT6D, K6A, Cytokeratin-6D, Cytokeratin-6A, CK-6D, CK-6A |
Ig Type | Rabbit IgG |
克隆号 | 4O729 |
交叉反应 | Human |
验证活性 | 1. Immunofluorescence staining of A549 with TMAH-00678 at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 519-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 2. Overlay Peak curve showing Hela cells stained with TMAH-00678 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
应用 | ELISA, IF, FC |
推荐剂量 | IF:1:50-1:200; FC:1:50-1:200. |
抗体种类 | Monoclonal |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human KRT6A |
抗原种属 | Human |
基因ID | 3853 |
Uniprot ID | |
研究领域 | Tags & Cell Markers, Signal transduction |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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