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Anti-KRT4 Antibody (5F548)
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产品编号 TMAC-01087
别名 Type-II keratin Kb4, type II cytoskeletal 4, KRT4, Keratin-4 (K4), Keratin, type II cytoskeletal 4, Cytokeratin-4 (CK-4), CYK4
Anti-KRT4 Antibody (5F548) 是一种 Rabbit 抗体，靶向 KRT4。Anti-KRT4 Antibody (5F548) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-KRT4 Antibody (5F548)
Anti-KRT4 Antibody (5F548)
还可以产品编号 TMAC-01087 别名 Type-II keratin Kb4, type II cytoskeletal 4, KRT4, Keratin-4 (K4), Keratin, type II cytoskeletal 4, Cytokeratin-4 (CK-4), CYK4
Anti-KRT4 Antibody (5F548) 是一种 Rabbit 抗体，靶向 KRT4。Anti-KRT4 Antibody (5F548) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-KRT4 Antibody (5F548) is a Rabbit antibody targeting KRT4. Anti-KRT4 Antibody (5F548) can be used in WB,ICC/IF,IHC,IP,FCM. |
| 别名 | Type-II keratin Kb4, type II cytoskeletal 4, KRT4, Keratin-4 (K4), Keratin, type II cytoskeletal 4, Cytokeratin-4 (CK-4), CYK4 |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 4 antibody. Counter stained with hematoxylin. 2. ICC staining Cytokeratin 4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Cytokeratin 4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Cytokeratin 4 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of A431 cells with Cytokeratin 4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Cytokeratins are a subfamily of intermediate filament keratins that are characterized by a remarkable biochemical diversity, which is represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in isoelectric range between 4.9 and 7.8. Cytokeratin 1 has the highest molecular weight, while Cytokeratin 19 has the lowest molecular weight. The cytokeratins are divided into the type I and type II subgroups. Type II family members comprise the basic to neutral members, Cytokeratins 1-8, while the type I group comprises the acidic members, Cytokeratins 9-20. Various epithelia in the human body usually express cytokeratins which are characteristic of the type of epithelium and related to the degree of maturation or differentiation within said epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. Cytokeratin 4 is expressed in differentiated layers of the mucosal and esophageal epithelia along with Cytokeratin 13. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 57 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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