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Anti-KRT18 Antibody (5J111)
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产品编号 TMAC-01083
别名 type I cytoskeletal 18, KRT18, Keratin-18 (K18), Keratin, type I cytoskeletal 18, Cytokeratin-18 (CK-18), CYK18, Cell proliferation-inducing gene 46 protein
Anti-KRT18 Antibody (5J111) 是一种 Rabbit 抗体，靶向 KRT18。Anti-KRT18 Antibody (5J111) 可用于 WB,ICC/IF,IHC,FCM。
Anti-KRT18 Antibody (5J111)
Anti-KRT18 Antibody (5J111)
还可以产品编号 TMAC-01083 别名 type I cytoskeletal 18, KRT18, Keratin-18 (K18), Keratin, type I cytoskeletal 18, Cytokeratin-18 (CK-18), CYK18, Cell proliferation-inducing gene 46 protein
Anti-KRT18 Antibody (5J111) 是一种 Rabbit 抗体，靶向 KRT18。Anti-KRT18 Antibody (5J111) 可用于 WB,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-KRT18 Antibody (5J111) is a Rabbit antibody targeting KRT18. Anti-KRT18 Antibody (5J111) can be used in WB,ICC/IF,IHC,FCM. |
| 别名 | type I cytoskeletal 18, KRT18, Keratin-18 (K18), Keratin, type I cytoskeletal 18, Cytokeratin-18 (CK-18), CYK18, Cell proliferation-inducing gene 46 protein |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Cytokeratin 18 on different lysates using anti-Cytokeratin 18 antibody at 1/20,000 dilution. Positive control: Lane 1: A431, Lane 2: Mouse colon, Lane 3: Mouse kidney. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin. 7. ICC staining Cytokeratin 18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Cytokeratin 18 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining Cytokeratin 18 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of MCF-7 cells with Cytokeratin 18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:10000-50000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, Cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while Cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Cytokeratin 18 contains two major phosphorylation sites on Ser 33 and Ser 52. Phosphorylation of Ser 18 is essential for the association of Cytokeratin 18 with 14-3-3 proteins and is involved in keratin organization and distribution. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 48 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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