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Anti-KDM1B Antibody (3V868)
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产品编号 TMAC-00221
别名 Lysine-specific histone demethylase 2, Lysine-specific histone demethylase 1B, LSD2, Flavin-containing amine oxidase domain-containing protein 1, C6orf193, AOF1
Anti-KDM1B Antibody (3V868) 是一种 Mouse 抗体，靶向 KDM1B。Anti-KDM1B Antibody (3V868) 可用于 WB,ICC,IHC,FCM。
Anti-KDM1B Antibody (3V868)
Anti-KDM1B Antibody (3V868)
还可以产品编号 TMAC-00221 别名 Lysine-specific histone demethylase 2, Lysine-specific histone demethylase 1B, LSD2, Flavin-containing amine oxidase domain-containing protein 1, C6orf193, AOF1
Anti-KDM1B Antibody (3V868) 是一种 Mouse 抗体，靶向 KDM1B。Anti-KDM1B Antibody (3V868) 可用于 WB,ICC,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,990 | 5日内发货 | |
| 100 μL | ¥ 2,990 | 5日内发货 |
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| 产品描述 | Anti-KDM1B Antibody (3V868) is a Mouse antibody targeting KDM1B. Anti-KDM1B Antibody (3V868) can be used in WB,ICC,IHC,FCM. |
| 别名 | Lysine-specific histone demethylase 2, Lysine-specific histone demethylase 1B, LSD2, Flavin-containing amine oxidase domain-containing protein 1, C6orf193, AOF1 |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of AOF1 on human AOF1 recombinant protein using anti-AOF1 antibody at 1/1,000 dilution. 2. Western blot analysis of AOF1 on HEK293 (1) and AOF1-hIgGFc transfected HEK293 (2) cell lysate using anti-AOF1 antibody at 1/1,000 dilution. 3. Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin. 5. ICC staining AOF1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela cells with AOF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:100-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | AOF1 (amine-oxidase flavin-containing domain 1), whose alternative names include KDM1B (lysine (K)-specific demethylase 1B) or LSD2 (lysine-specific histone demethylase 2), is an 823 amino acid nuclear protein belonging to the flavin monoamine oxidase family. As a histone demethylase, AOF1 specifically demethylates Lys4 of histone H3, a marker for epigenetic transcriptional activation, by interacting with a long stretch of the H3 N-terminal tail of mono- and dimethylated Lys4. Existing as three alternatively spliced isoforms, AOF1 is required for de novo DNA methylation of some imprinted genes in oocytes and contains a single SWIRM domain that is implicated in chromatin regulation. AOF1 contains one CW-type zinc finger and is encoded by a gene located on human chromosome 6p22.3. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 92 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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