Anti-ITGAE Polyclonal Antibody is a Rabbit antibody targeting ITGAE. Anti-ITGAE Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Rabbit)

1. Sample:
LympHnode (Mouse) Lysate at 40 μg
Primary: Anti-Integrin alpha E2 (TMAB-00993) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110/128 kDa
Observed band size: 128 kDa
2. Sample:
U937 (Human) Cell Lysate at 30 μg
Raji (Human) Cell Lysate at 30 μg
Primary: Anti-Integrin alpha E2 (TMAB-00993) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110/128 kDa
Observed band size: 110 kDa
3. Molt-4 cells were fixed with 4% PFA for 10 min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD103 Antibody (TMAB-00993) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

WB: 1:500-2000; FCM: 1ug/test

Polyclonal

KLH conjugated synthetic peptide: human Integrin alpha-E heavy chain

Human

Regulatory T Cells,Adhesion,Alpha,T Lymphocytic Lineage