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Anti-ITCH/AIP4 Antibody (6J974)
还可以产品编号 TMAY-01671
别名 NAPP1, ITCH, AIP4, AIP 4, AIF4, ADMFD
Anti-ITCH/AIP4 Antibody (6J974) 是一种 Mouse 抗体，靶向 ITCH/AIP4。Anti-ITCH/AIP4 Antibody (6J974) 可用于 FCM,ICC/IF。
Anti-ITCH/AIP4 Antibody (6J974)
Anti-ITCH/AIP4 Antibody (6J974)
还可以产品编号 TMAY-01671 别名 NAPP1, ITCH, AIP4, AIP 4, AIF4, ADMFD
Anti-ITCH/AIP4 Antibody (6J974) 是一种 Mouse 抗体，靶向 ITCH/AIP4。Anti-ITCH/AIP4 Antibody (6J974) 可用于 FCM,ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 1,689 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-ITCH/AIP4 Antibody (6J974) is a Mouse antibody targeting ITCH/AIP4. Anti-ITCH/AIP4 Antibody (6J974) can be used in FCM,ICC/IF. |
| 别名 | NAPP1, ITCH, AIP4, AIP 4, AIF4, ADMFD |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 6J974 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human ITCH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ITCH monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human ITCH expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human ITCH, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ITCH / AIP4 (rh ITCH / AIP4; NP_113671.3; Arg526-Glu903). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | E3 ubiquitin-protein ligase Itchy homolog, also known as Atrophin-1-interacting protein 4, NFE2-associated polypeptide 1, NAPP1, and ITCH, is a cell membrane protein that contains one C2 domain, one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and contains four WW domains. ITCH acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. ITCH is involved in the control of inflammatory signaling pathways. It is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1, and RNF11, that ensures the transient nature of inflammatory signaling pathways. ITCH promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Defects in ITCH are the cause of the syndromic multisystem autoimmune disease (SMAD) which is characterized by organomegaly, failure to thrive, developmental delay, dysmorphic features, and autoimmune inflammatory cell infiltration of the lungs, liver, and gut. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | FITC |
| 可提供抗体种类 | 2 |
| 免疫原 | Recombinant Human ITCH / AIP4 protein |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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