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Anti-IRF5 Antibody (2A61) 是一种 Rabbit 抗体,靶向 IRF5。Anti-IRF5 Antibody (2A61) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-IRF5 Antibody (2A61) 是一种 Rabbit 抗体,靶向 IRF5。Anti-IRF5 Antibody (2A61) 可用于 WB,ICC/IF,IHC,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-IRF5 Antibody (2A61) is a Rabbit antibody targeting IRF5. Anti-IRF5 Antibody (2A61) can be used in WB,ICC/IF,IHC,IP,FCM. |
别名 | IRF-5, IRF5, Interferon Regulatory Factor 5 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of IRF5 on THP-1 cells lysates using anti-IRF5 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IRF5 antibody. Counter stained with hematoxylin. 3. ICC staining IRF5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining IRF5 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Hela cells with IRF5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Interferon regulatory factor 5 (IRF-5), belongs to the IRF family of DNA-binding factors, which includes IRF-1, IRF-2, IRF-3, IRF-4, IRF-6, IRF-7, ISGF-3γ p48 and IFN consensus sequence-binding protein (ICSBP). The IRF family regulate both interferon and interferon-inducible genes. IRF-5, like IRF-3 and IRF-7, is a direct transducer of virus-mediated signaling and plays a role in the expression of multiple cytokines/chemokines. Although IRF-5 is a direct target of p53, its cell cycle regulatory and proapoptotic effects are p53 independent. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 56 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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