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Anti-Intrinsic Factor Antibody (1E668)
还可以产品编号 TMAY-02597
Anti-Intrinsic Factor Antibody (1E668) 是一种 Rabbit 抗体，靶向 Intrinsic Factor。Anti-Intrinsic Factor Antibody (1E668) 可用于 FCM,ICC/IF。
Anti-Intrinsic Factor Antibody (1E668)
Anti-Intrinsic Factor Antibody (1E668)
还可以产品编号 TMAY-02597
Anti-Intrinsic Factor Antibody (1E668) 是一种 Rabbit 抗体，靶向 Intrinsic Factor。Anti-Intrinsic Factor Antibody (1E668) 可用于 FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Intrinsic Factor Antibody (1E668) is a Rabbit antibody targeting Intrinsic Factor. Anti-Intrinsic Factor Antibody (1E668) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 1E668 |
| 交叉反应 | Human |
| 验证活性 | 1. Flow cytometric analysis of Human GIF expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human GIF, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of GIF in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human GIF monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Gastric intrinsic factor / GIF (rh Gastric intrinsic factor / GIF; TMPY-03221; P27352-1; Met1-Tyr417). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Gastric intrinsic factor / GIF Protein (TMPY-03221) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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