Anti-Integrin alpha 3 beta 1 Polyclonal Antibody is a Rabbit antibody targeting Integrin alpha 3 beta 1. Anti-Integrin alpha 3 beta 1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Sheep)

1. Sample: Cerebrum (Mouse) Lysate at 30 μg
Primary: Anti-Integrin Alpha 3 + Beta 1 (TMAB-00975) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 117 kDa
2. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Integrin Alpha 3 + Beta 1 Polyclonal Antibody, Unconjugated (TMAB-00975) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample: Lung (Mouse) Lysate at 30 μg
Primary: Anti-Integrin Alpha 3 + Beta 1 (TMAB-00975) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 130 kDa
4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Integrin Alpha 3 + Beta 1) Polyclonal Antibody, Unconjugated (TMAB-00975) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Blank control: A431. Primary Antibody (green line): Rabbit Anti-Integrin Alpha 3 + Beta 1 antibody (TMAB-00975)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human Integrin Alpha 3(976-1025aa) + Beta 1(35-47aa)

Human

Alpha