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Anti-INCENP Antibody (3Z349)
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产品编号 TMAC-02158
别名 Inner centromere protein INCENP, Inner centromere protein antigens 135kD 155kD, Inner centromere protein antigens 135/155kDa, Inner centromere protein, INCENP, INCE_HUMAN, Chromosomal passenger protein, binds and activates aurora B and C in vivo and in vitro
Anti-INCENP Antibody (3Z349) 是一种 Mouse 抗体，靶向 INCENP。Anti-INCENP Antibody (3Z349) 可用于 WB,ICC,IHC,FCM。
Anti-INCENP Antibody (3Z349)
Anti-INCENP Antibody (3Z349)
还可以产品编号 TMAC-02158 别名 Inner centromere protein INCENP, Inner centromere protein antigens 135kD 155kD, Inner centromere protein antigens 135/155kDa, Inner centromere protein, INCENP, INCE_HUMAN, Chromosomal passenger protein, binds and activates aurora B and C in vivo and in vitro
Anti-INCENP Antibody (3Z349) 是一种 Mouse 抗体，靶向 INCENP。Anti-INCENP Antibody (3Z349) 可用于 WB,ICC,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,980 | 5日内发货 | |
| 100 μL | ¥ 2,980 | 5日内发货 |
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| 产品描述 | Anti-INCENP Antibody (3Z349) is a Mouse antibody targeting INCENP. Anti-INCENP Antibody (3Z349) can be used in WB,ICC,IHC,FCM. |
| 别名 | Inner centromere protein INCENP, Inner centromere protein antigens 135kD 155kD, Inner centromere protein antigens 135/155kDa, Inner centromere protein, INCENP, INCE_HUMAN, Chromosomal passenger protein, binds and activates aurora B and C in vivo and in vitro |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of INCENP on human INCENP recombinant protein using anti-INCENP antibody at 1/1,000 dilution. 2. Western blot analysis of INCENP on HEK293 (1) and INCENP-hIgGFc transfected HEK293 (2) cell lysate using anti-INCENP antibody at 1/1,000 dilution. 3. Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin. 5. ICC staining INCENP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Jurkat cells with INCENP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:100-500; FCM: 1:100-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
| 研究背景 | A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The centromere proteins CENP-A, CENP-B, CENP-C, CENP-E, CENP-F (also designated mitosin), CENP-H and INCENP are kinetochore proteins that are involved in mitotic events. The centromere proteins are expressed at different levels throughout the cell cycle and are involved in the formation of the centromere and the organization and function of the kinetochore. INCENP, which also is designated inner centromere protein, is a chromosomal passenger protein that is crucial for chromosome segregation. During mitosis it is also required for cytokinesis onset. This protein, which can form a homodimer or a heterodimer, binds directly to microtubules and interacts with AURKB, AURKC, CBX3 and β Tubulin. This nuclear protein localizes to the mitotic spindle, methaphase chromosomes and during anaphase, to the equatorial cortex. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 105 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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