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Anti-ILF3 Antibody (8T791)

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Anti-ILF3 Antibody (8T791)
产品编号 TMAC-02153
别名 Translational control protein 80, TCP80, Nuclear factor of activated T-cells 90 kDa, Nuclear factor associated with dsRNA, NF-AT-90, NFAR, MPP4, M-phase phosphoprotein 4, Interleukin enhancer-binding factor 3, ILF 3, DRBP76, DRBF MPHOSPH4 NF90, Double-stranded RNA-binding protein 76

Anti-ILF3 Antibody (8T791) 是一种 Rabbit 抗体，靶向 ILF3。Anti-ILF3 Antibody (8T791) 可用于 WB,IP,ICC,IF,IHC,FCM。

Anti-ILF3 Antibody (8T791)

Anti-ILF3 Antibody (8T791)

Rating icon 还可以
Anti-ILF3 Antibody (8T791)
产品编号 TMAC-02153 别名 Translational control protein 80, TCP80, Nuclear factor of activated T-cells 90 kDa, Nuclear factor associated with dsRNA, NF-AT-90, NFAR, MPP4, M-phase phosphoprotein 4, Interleukin enhancer-binding factor 3, ILF 3, DRBP76, DRBF MPHOSPH4 NF90, Double-stranded RNA-binding protein 76

Anti-ILF3 Antibody (8T791) 是一种 Rabbit 抗体，靶向 ILF3。Anti-ILF3 Antibody (8T791) 可用于 WB,IP,ICC,IF,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-ILF3 Antibody (8T791) is a Rabbit antibody targeting ILF3. Anti-ILF3 Antibody (8T791) can be used in WB,IP,ICC,IF,IHC,FCM.
别名Translational control protein 80, TCP80, Nuclear factor of activated T-cells 90 kDa, Nuclear factor associated with dsRNA, NF-AT-90, NFAR, MPP4, M-phase phosphoprotein 4, Interleukin enhancer-binding factor 3, ILF 3, DRBP76, DRBF MPHOSPH4 NF90, Double-stranded RNA-binding protein 76
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of ILF3 on rat heart tissue (1) and 293 cell (2) lysate using anti-ILF3 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
6. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
7. ICC staining ILF3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining ILF3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. ICC staining ILF3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
10. Flow cytometric analysis of K562 cells with ILF3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
应用
WB
IP
ICC
IF
IHC
FCM
推荐剂量
WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景ILF3 (interleukin enhancer binding factor 3) or TCP80 (translational control protein 80), is a ubiquitously expressed nuclear protein that exists in a heterodimer with NF45. NF90 contains two DRBM (double-stranded RNA(dsRNA-)-binding) domains and one DZF domain and, in association with NF45 primarily participates in the regulation of IL-2 expression by binding to the antigen receptor response element (ARRE) target sequence of the IL-2 enhancer. In neuronal cells, the NF45/NF90 heterodimer can repress human rhinovirus type 2 replication by binding to a 5' untranslated region of the viral RNA that encodes the internal ribosome entry site (IRES). NF45 and NF90 belong to the double-stranded RNA-binding protein family and both are substrates for the dsRNA-activated protein kinase, PKR. Due to alternative splicing events, six isoforms exist for NF90, namely NFAR-2 (or ILF3-E), NFAR-1 (or DRBP76), isoform 3, DRBP76α (or ILF3-A), DRBP76δ (also known as DRBP76γ or ILF3-C) and isoform 6.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: N-terminal human ILF3
抗原种属
human
Uniprot ID
Q12906
化学信息
分子量Theoretical: 75/95 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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