Anti-IL2RA/CD25 Antibody (3V25) is a Mouse antibody targeting IL2RA/CD25. Anti-IL2RA/CD25 Antibody (3V25) can be used in FCM.

IgG2a, κ

3V25

Human

1. scatter diagram showing peripheral blood lymphocytes stained with CD25. The cells were incubated with the antibody (TMAB-07652) for 30 min at 22°C. The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.
2. Blank control: sR.
Primary Antibody (green line): Mouse Anti-CD25 antibody (TMAB-07652)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line) : Goat anti-mouse IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Monoclonal

KLH conjugated synthetic peptide: human IL2RA/CD25

Human

IL2RA

Interleukin-2 receptor subunit alpha

Receptor for interleukin-2.