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Anti-IL-1 alpha/IL-1A Polyclonal Antibody
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产品编号 TMAB-00931
Anti-IL-1 alpha/IL-1A Polyclonal Antibody 是一种 Rabbit 抗体，靶向 IL-1 alpha/IL-1A。Anti-IL-1 alpha/IL-1A Polyclonal Antibody 可用于 WB,FCM。
Anti-IL-1 alpha/IL-1A Polyclonal Antibody
Anti-IL-1 alpha/IL-1A Polyclonal Antibody
还可以产品编号 TMAB-00931
Anti-IL-1 alpha/IL-1A Polyclonal Antibody 是一种 Rabbit 抗体，靶向 IL-1 alpha/IL-1A。Anti-IL-1 alpha/IL-1A Polyclonal Antibody 可用于 WB,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-IL-1 alpha/IL-1A Polyclonal Antibody is a Rabbit antibody targeting IL-1 alpha/IL-1A. Anti-IL-1 alpha/IL-1A Polyclonal Antibody can be used in WB,FCM. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse (predicted:Rat) |
| 验证活性 | 1. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-IL-1 Alpha (TMAB-00931) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/31 kDa Observed band size: 31 kDa 2. Sample: Lver (Mouse) Lysate at 40 μg Primary: Anti-IL-1 Alpha (TMAB-00931) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/31 kDa Observed band size: 31 kDa 3. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-IL-1 Alpha antibody (TMAB-00931) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; FCM: 1ug/Test |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence. |
| 免疫原 | KLH conjugated synthetic peptide: rat IL-1 Alpha |
| 抗原种属 | Rat |
| 基因ID | |
| Uniprot ID |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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