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Anti-IkB alpha/NFKBIA Polyclonal Antibody

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Anti-IkB alpha/NFKBIA Polyclonal Antibody
产品编号 TMAB-00928
别名 nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α

Anti-IkB alpha/NFKBIA Polyclonal Antibody 是一种 Rabbit 抗体,靶向 IkB alpha/NFKBIA。Anti-IkB alpha/NFKBIA Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM。

Anti-IkB alpha/NFKBIA Polyclonal Antibody

Anti-IkB alpha/NFKBIA Polyclonal Antibody

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Anti-IkB alpha/NFKBIA Polyclonal Antibody
产品编号 TMAB-00928 别名 nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α

Anti-IkB alpha/NFKBIA Polyclonal Antibody 是一种 Rabbit 抗体,靶向 IkB alpha/NFKBIA。Anti-IkB alpha/NFKBIA Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,795
5日内发货
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实验操作小课堂
常见问题解答
化合物带有盐酸盐离子、硫酸盐离子等是否和其本身有什么区别?盐形式和游离态有什么区别?
盐和非盐形式化合物的活性分子是一样的,在生物实验中起到的效果也一致,活性和使用方法都是一样的。只是由于呈盐不同,物理性质比如溶解度会有差异。建议您根据溶解、实验需求进行选择。
如何选择某个靶点的特异性或总的抑制剂?特异性和非特异性的区别是什么?
抑制剂按照特异性分为广谱 pan 和特异性 selective 两种。Pan 为某个靶点总的抑制剂,对所有亚型或整个家族的成员都有抑制作用。Selective 抑制剂针对某个蛋白激酶的某个亚型或家族中某个成员抑制率特别高或有特异性抑制作用。 一般评价一个抑制剂的抑制效率主要看 IC50 值,IC50 值越低,说明抑制剂效率越高。建议您根据以上几个特征进行综合选择,也可联系技术帮您推荐相关抑制剂。
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产品介绍

生物活性
产品描述
Anti-IkB alpha/NFKBIA Polyclonal Antibody is a Rabbit antibody targeting IkB alpha/NFKBIA. Anti-IkB alpha/NFKBIA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
别名nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-IKB alpha Polyclonal Antibody, Unconjugated (TMAB-00928) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control (blue line): Jurkat (blue).
Primary Antibody (green line): Rabbit Anti-IKB alpha antibody (TMAB-00928)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 μg
Lane 2: Hela (Human) Cell Lysate at 30 μg
Lane 3: Siha (Human) Cell Lysate at 30 μg
Lane 4: U251 (Human) Cell Lysate at 30 μg
Lane 5: Spleen (Mouse) Lysate at 40 μg
Lane 6: Thymus (Mouse) Lysate at 40 μg
Lane 7: Thymus (Rat) Lysate at 40 μg
Lane 8: LympHnode (Mouse) Lysate at 40 μg
Lane 9: LympHnode (Rat) Lysate at 40 μg
Primary: Anti-IKB alpha (TMAB-00928) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 37 kDa
4. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-IKB alpha antibody (TMAB-00928)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (IKB alpha) polyclonal Antibody, Unconjugated (TMAB-00928) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
6. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IKB alpha) Polyclonal Antibody, Unconjugated (TMAB-00928) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IKB alpha) Polyclonal Antibody, Unconjugated (TMAB-00928) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1μg/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
抗原信息
免疫原
KLH conjugated synthetic peptide: human NFKBIA
抗原种属
Human
基因ID
Uniprot ID
研究领域
NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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