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Anti-IkB alpha/NFKBIA Polyclonal Antibody 是一种 Rabbit 抗体,靶向 IkB alpha/NFKBIA。Anti-IkB alpha/NFKBIA Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM。
Anti-IkB alpha/NFKBIA Polyclonal Antibody 是一种 Rabbit 抗体,靶向 IkB alpha/NFKBIA。Anti-IkB alpha/NFKBIA Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,965 | 5日内发货 | |
200 μL | ¥ 2,795 | 5日内发货 |
产品描述 | Anti-IkB alpha/NFKBIA Polyclonal Antibody is a Rabbit antibody targeting IkB alpha/NFKBIA. Anti-IkB alpha/NFKBIA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
别名 | nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-IKB alpha Polyclonal Antibody, Unconjugated (TMAB-00928) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Blank control (blue line): Jurkat (blue). Primary Antibody (green line): Rabbit Anti-IKB alpha antibody (TMAB-00928) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1 μg/test. Protocol The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. 3. Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 μg Lane 2: Hela (Human) Cell Lysate at 30 μg Lane 3: Siha (Human) Cell Lysate at 30 μg Lane 4: U251 (Human) Cell Lysate at 30 μg Lane 5: Spleen (Mouse) Lysate at 40 μg Lane 6: Thymus (Mouse) Lysate at 40 μg Lane 7: Thymus (Rat) Lysate at 40 μg Lane 8: LympHnode (Mouse) Lysate at 40 μg Lane 9: LympHnode (Rat) Lysate at 40 μg Primary: Anti-IKB alpha (TMAB-00928) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 37 kDa 4. Blank control: Hela. Primary Antibody (green line): Rabbit Anti-IKB alpha antibody (TMAB-00928) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 5. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (IKB alpha) polyclonal Antibody, Unconjugated (TMAB-00928) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 6. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IKB alpha) Polyclonal Antibody, Unconjugated (TMAB-00928) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IKB alpha) Polyclonal Antibody, Unconjugated (TMAB-00928) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1μg/Test |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
免疫原 | KLH conjugated synthetic peptide: human NFKBIA |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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