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Anti-IGF1R/CD221 Polyclonal Antibody 2
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产品编号 TMAB-00919
Anti-IGF1R/CD221 Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 IGF1R/CD221。Anti-IGF1R/CD221 Polyclonal Antibody 2 可用于 WB,FCM。
Anti-IGF1R/CD221 Polyclonal Antibody 2
Anti-IGF1R/CD221 Polyclonal Antibody 2
还可以产品编号 TMAB-00919
Anti-IGF1R/CD221 Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 IGF1R/CD221。Anti-IGF1R/CD221 Polyclonal Antibody 2 可用于 WB,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-IGF1R/CD221 Polyclonal Antibody 2 is a Rabbit antibody targeting IGF1R/CD221. Anti-IGF1R/CD221 Polyclonal Antibody 2 can be used in WB,FCM. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse (predicted:Rat,Pig,Cow,Sheep) |
| 验证活性 | 1. Positive control: (mo) splenocytes (2% Paraformaldehyde-fixed) Isotype Control Antibody: Rabbit IgG; Dilution: 1 μg in 100 μL 1 X PBS containing 0.5% BSA Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody: rabbit Anti-IGF1R TMAB-00919; Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA 2. Blank control (blue): Mouse Spleen (fixed with 2% paraformaldehyde for 10 min at 37°C). Primary Antibody: Rabbit Anti-IGF1R antibody (TMAB-00919,Green); Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. 3. Sample: Kidney (Mouse) Lysate at 40 μg Primary: Anti-IGF1R (TMAB-00919) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69/150 kDa Observed band size: 69 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; FCM: 1μg/Test |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin. |
| 免疫原 | KLH conjugated synthetic peptide: human IGF1R/CD221 beta chain |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Insulin / Insulin-like,Aging,Insulin and insulin-like |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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