Anti-IGF1R/CD221 Polyclonal Antibody is a Rabbit antibody targeting IGF1R/CD221. Anti-IGF1R/CD221 Polyclonal Antibody can be used in FCM.

IgG

Human,Mouse,Dog (predicted:Rat,Pig,Cow,Horse,Rabbit,Sheep)

1. Positive control: (mo) splenocytes (2% Paraformaldehyde-fixed)
Isotype Control Antibody
Antibody: Rabbit IgG;
Supplier: Cell Signaling Technology;
Dilution: 1 μg in 100 μL 1 X PBS containing 0.5% BSA
Secondary Antibody
Antibody: Goat anti-rabbit IgG-FITC;
Supplier: Cell Signaling Technology;
Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody
Supplier: Bioss;
Supplier catalog number: TMAB-00918;
Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA
2. Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-IGF1R antibody (TMAB-00918)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human IGF-1R alpha chain

Human

Insulin / Insulin-like,Aging,Insulin and insulin-like