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Anti-IFI30 Antibody (4Q235)
还可以产品编号 TMAY-02400
Anti-IFI30 Antibody (4Q235) 是一种 Rabbit 抗体，靶向 IFI30。Anti-IFI30 Antibody (4Q235) 可用于 IHC-P,ICC/IF。
Anti-IFI30 Antibody (4Q235)
Anti-IFI30 Antibody (4Q235)
还可以产品编号 TMAY-02400
Anti-IFI30 Antibody (4Q235) 是一种 Rabbit 抗体，靶向 IFI30。Anti-IFI30 Antibody (4Q235) 可用于 IHC-P,ICC/IF。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-IFI30 Antibody (4Q235) is a Rabbit antibody targeting IFI30. Anti-IFI30 Antibody (4Q235) can be used in IHC-P,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 4Q235 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunochemical staining of human IFI30 in human tonsil with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of human IFI30 in human lung with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections). 3. Immunofluorescence staining of IFI30 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- IFI30 monoclonal antibody (dilution ratio 1:30) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm. |
| 应用 | IHC-P,ICC/IF |
| 推荐剂量 | IHC-P: 1:10000-1:20000; ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human IFI30 (rh IFI30; TMPY-02887; P13284; Met1-Lys243). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | IFI30 belongs to the GILT family. This family includes the two characterized human gamma-interferon-inducible lysosomal thiol reductase (GILT) sequences: P13284 and Q9UL08. It also contains several other eukaryotic putative proteins with similarity to GILT. The aligned region contains three conserved cysteine residues. Besides, the two GILT sequences possess a C-X(2)-C motif that is shared by some of the other sequences in the family. This motif is thought to be associated with disulfide bond reduction. IFI30 is a lysosomal thiol reductase that can reduce protein disulfide bonds. It facilitates the generation of MHC class II-restricted epitopes from disulfide bond-containing antigens by the endocytic reduction of disulfide bonds. It also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation. IFI30 may facilitate the complete unfolding of proteins destined for lysosomal degradation and plays an important role in antigen processing. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human IFI30 Protein (TMPY-02887) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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