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顶部生物活性储存和溶解信息计算器

Anti-ID1 Antibody (6Z914)

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Anti-ID1 Antibody (6Z914)
产品编号 TMAC-02084
别名 bHLHb24

Anti-ID1 Antibody (6Z914) 是一种 Rabbit 抗体，靶向 ID1。Anti-ID1 Antibody (6Z914) 可用于 WB,ICC/IF,IHC,FCM。

Anti-ID1 Antibody (6Z914)

Anti-ID1 Antibody (6Z914)

Rating icon 还可以
Anti-ID1 Antibody (6Z914)
产品编号 TMAC-02084 别名 bHLHb24

Anti-ID1 Antibody (6Z914) 是一种 Rabbit 抗体，靶向 ID1。Anti-ID1 Antibody (6Z914) 可用于 WB,ICC/IF,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-ID1 Antibody (6Z914) is a Rabbit antibody targeting ID1. Anti-ID1 Antibody (6Z914) can be used in WB,ICC/IF,IHC,FCM.
别名bHLHb24
Ig Type
IgG
交叉反应
Human
验证活性
1. Western blot analysis of Id1 on 293T cell using anti-Id1 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Id1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Id1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Id1 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Id1 antibody. Counter stained with hematoxylin.
6. ICC staining Id1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Id1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining Id1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of HepG2 cells with Id1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
应用
WB
ICC/IF
IHC
FCM
推荐剂量
WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P41134
化学信息
分子量Theoretical: 22 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

Inhibitor of DNA binding 1Inhibitor of differentiation 1ID1ID 1IDDNA-binding protein inhibitor ID-1Class B basic helix-loop-helix protein 24 (bHLHb24)bHLHb 24

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