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Anti-ICAM-1/CD54 Antibody (4H175)
还可以产品编号 TMAY-00663
Anti-ICAM-1/CD54 Antibody (4H175) 是一种 Mouse 抗体，靶向 ICAM-1/CD54。Anti-ICAM-1/CD54 Antibody (4H175) 可用于 ELISA,ELISA(Cap),FCM,ICC/IF。
Anti-ICAM-1/CD54 Antibody (4H175)
Anti-ICAM-1/CD54 Antibody (4H175)
还可以产品编号 TMAY-00663
Anti-ICAM-1/CD54 Antibody (4H175) 是一种 Mouse 抗体，靶向 ICAM-1/CD54。Anti-ICAM-1/CD54 Antibody (4H175) 可用于 ELISA,ELISA(Cap),FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-ICAM-1/CD54 Antibody (4H175) is a Mouse antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody (4H175) can be used in ELISA,ELISA(Cap),FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 4H175 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human ICAM1 in MSC cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with Mouse anti-Human ICAM1 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane. 2. Flow cytometric analysis of Human ICAM1 (CD54) in HUVEC cells. HUVEC cells (Human umbilical vein endothelial cells) were either treated with TNF-α (50ng/ml,18 hours) (right panel) or untreated (left panel). The cells were harvested and stained with purified human ICAM1, then a FITC-conjugated second step antibody. |
| 应用 | ELISA,ELISA(Cap),FCM,ICC/IF |
| 推荐剂量 | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM1 / CD54 (rh ICAM1 / CD54; TMPY-01142; Met 1-Glu 480; NP_000192.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. ved. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/PerCP/FITC/PE |
| 可提供抗体种类 | 5 |
| 免疫原 | Recombinant Human ICAM-1 protein (TMPY-01142) |
| 抗原种属 | Human |
| 研究领域 | Neuroinflammation |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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